Journal: bioRxiv
Article Title: Rapid and precise quantification of lymphocyte iron content by single cell inductively coupled plasma mass spectrometry
doi: 10.1101/2024.11.11.623006
Figure Lengend Snippet: (A) . Experimental design for the assessment of iron in murine T-cells that were exposed to iron media conditions ranging from 0.001mg/mL – 0.625mg/mL holotransferrin; (B). Histograms showing the mass-frequency distributions of iron mass/cell for each holotransferrin condition from cells taken from each of three mice. Mass distributions are shown in attograms (ag) (10 -18 g); (C). Line plots showing the mean iron mass/cell per tenth percentile for each holotransferrin condition from each mouse; (D). Line plots presenting proportions of cell proliferation activity per iron condition (percentage of total cells dividing 0, 1 or 2+ times) with log 10 x-axes from each mouse; (E). Correlation plot presenting the geometric means from each distribution versus the iron condition for each mouse (with a log 10 x-axis). Logarithmic trend lines, associated equations and r 2 values are displayed for each profile. Error bars show the 95 th confidence intervals; (F). Line plot presenting the mean total amount of iron consumed by the cells from the three mice for each iron condition (with a log 10 x-axis). Error bars show the range of total iron calculated per mouse. (G). Scatter plot presenting the inverse correlation between the magnitude of cell proliferation during the live culturing period against the skewness of the single cell metallomic distributions for iron.
Article Snippet: Human holotransferrin (R&D systems, 2914-HT-001G) was added at concentrations of 0.001 mg/mL to 0.625 mg/mL.
Techniques: Activity Assay
